Tandem Affinity Purification/ MS has not posted anything yet

Tandem affinity purification (TAP) method is a tool that allows rapid purification protein complex under native conditions, even when expressed at their natural level. The method involves the fusion of the TAP tag to the protein and two-step affinity purification process. An advantage of this method is that there can be real determination of protein partners quantitatively in vivo without prior knowledge of complex composition. Its simplicity, high yield, and wide applicability make it a very useful procedure for protein purification and proteome exploration.